Methodology: The study design was descriptive non-experimental design. The objectives of the study were to assess the knowledge and attitude of postnatal mothers on human milk banking & to find the association between level of knowledge, attitude on human milk banking and socio demographic variables.100 post natal mothers was selected for the study by using Non-probability purposive sampling technique.

Result: The major finding showed that the majority of 48(48%) having poor knowledge regarding human milk banking, 35(35%) postnatal mother having average knowledge on human milk banking and 17(17%) of postnatal mothers having poor knowledge on human milk banking. majority of 40% postnatal mothers are showed unfavourable Attitude There is a significant association between demographic variables (age and religion of postnatal mothers) and level of knowledge on human milk banking. But there was no significant association between educations, occupation, type of family, previous information with level of knowledge on human milk banking. there is a significant association between demographic variables (education, and attitude of postnatal mothers on human milk banking). But there was no significant association between age, religion, occupation and type of family with the attitude of postnatal mothers on human milk banking.

Conclusion: This indicates that majority of the postnatal mothers had poor knowledge and attitude towards human milk banking. Therefore it is necessary for health professionals especially nurses to encourage mothers to continue breast feeding and also to create awareness about human milk banking.