A cross sectional non experimental descriptive study was carried out to assess the knowledge and practice regarding Post-Partum Haemorrhage (PPH) prevention and its management among 130 nurses (65 nurses from rural health care facilities and 65 from urban health care facilities) working in selected rural and urban Health care facilities, Meghalaya using multistage sampling technique. Majority of nurses, particularly in urban settings, demonstrated good knowledge levels (83.1%). Rural nurses showed satisfactory practice (67.7%), both rural and urban nurses exhibited similar practice scores. There is a significant difference between knowledge score of the nurse in rural and urban health care facilities at (t (128) = -2.4, p=0.02*) in the rural knowledge score with mean and standard deviation (Mean ± SD) is 17±2.7, while the urban group had a mean and standard deviation of knowledge score (Mean ± SD) is 18.1±2.3 at 95% confidence interval of difference. Whereas there is no statistical significant difference between the practice score of the nurses in the rural and urban health care facilities. Logistic regression revealed associations between unsatisfactory practice levels and reduced knowledge in rural settings, emphasizing the impact of age on practice. In urban areas, unsatisfactory practice levels are associated with lower odds of good knowledge. These results also highlight the importance of addressing and improving practice levels to enhance knowledge and effectively manage and prevent Post-Partum Hemorrhage (PPH) among nurses in both rural and urban settings.Conclusion: The study underscores the global importance of implementing health interventions to prevent and treat PPH complications, enhancing maternal health outcomes.