Background: Cervical cancer is the second most common cancer in women worldwide, mainly caused by Human Papilloma Virus (HPV)—the most common sexually transmitted infection, affecting about 80% of sexually active individuals. HPV is linked to cancers of the cervix, throat, mouth, anus, penis, and vagina. Prevention includes HPV testing, vaccination, and use of barrier methods.

Methodology: assess knowledge regarding HPV vaccination among undergraduate students, A Quantitative research approach with pre-experimental design one group pre-test and post-test was adopted for this study on 60 undergraduate students of selected colleges age between 17-25 years. Using non – probability purposive sampling technique. The Data were collected with a self-structured questionnaires and variable include Age, Marital status, Place of residence, Family history of any cancer, Types of lifestyle, Age of Menarche, Received HPV vaccination, and Sources of information. Following the pre-test, structured teaching programme was administered and post-test was conducted. The obtained data was analysed in terms of the objectives and hypothesis using descriptive and inferential statistics technique.

Results: Results showed that in the pre-test, only 1.67% had adequate knowledge, 13.33% moderately adequate, and 85% inadequate knowledge. In the post-test, 48.33% had adequate knowledge, 43.33% moderately adequate, and only 8.33% inadequate knowledge. The mean knowledge score increased from 6.88 (SD=4.35) to 20.13 (SD=4.83), with a mean difference of 13.25. The obtained t value (19.24) was statistically significant at p<0.05, indicating the intervention significantly improved knowledge; hence, the null hypothesis was rejected.

Conclusion: structured teaching programme helps undergraduate students to take proper knowledge regarding HPV vaccination.